Insights into the Mechanism of Pore Opening of Acid-sensing Ion Channel 1A
نویسندگان
چکیده
منابع مشابه
Insights into the mechanism of pore opening of acid-sensing ion channel 1a.
Acid-sensing ion channels (ASICs) are trimeric cation channels that undergo activation and desensitization in response to extracellular acidification. The underlying mechanism coupling proton binding in the extracellular region to pore gating is unknown. Here we probed the reactivity toward methanethiosulfonate (MTS) reagents of channels with cysteine-substituted residues in the outer vestibule...
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Objective(s): Activation of acid-sensing ion channel 1a (ASIC1a) is responsible for tissue injury caused by acidosis in nervous systems. But its physiological and pathological roles in nucleus pulposus cells (NPCs) are unclear. The aim of this study is to investigate whether ASIC1a regulates the survival of NPCs in the acidic environment of degenerated discs. Materials and Methods: NPCs were i...
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Although acid-sensitive ion channels (ASICs) play an important role in brain functions, the exact mechanism of their physiological activation remain unclear. A possible answer to the intriguing question is that some presently unknown endogenous ligand(s) positively modulate ASICs and enhance their responses to physiologically significant level. In the present work we found that histamine select...
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objective(s): activation of acid-sensing ion channel 1a (asic1a) is responsible for tissue injury caused by acidosis in nervous systems. but its physiological and pathological roles in nucleus pulposus cells (npcs) are unclear. the aim of this study is to investigate whether asic1a regulates the survival of npcs in the acidic environment of degenerated discs. materials and methods: npcs were is...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2011
ISSN: 0021-9258
DOI: 10.1074/jbc.m110.202366